Table 1 Plasmids used in the study
From: Efficient generation of influenza virus with a mouse RNA polymerase I-driven all-in-one plasmid
Plasmid type | Plasmid (related properties) | ori and marker |
|---|---|---|
Vector plasmid | pcDNA3.1(−) (CMV, SV40 pA) | pUC ori, ApR |
T-vector precursor | pYA4518 (AhdI-GFP cassette-AhdI) | p15A ori, CmR |
Reporter plasmid | pYA4392 (CPI-EGFP), pYA4924 (MPI-EGFP) | |
pYA4332 (GFP cassette) | pUCori, ApR | |
pYA4731 (CMV-mCherry) | ||
pYA4732 (CMV-mCherry, PB2, PB1, PA, NP, HA, NA, M, NS) | p15A ori, CmR; | |
MPI-based bidirectional vector | pYA4963 (CMV-MPI), pYA4964 (CMV-GFP cassette-MPI) | pUCori, ApR |
CPI-based 8-plasmid system | pYA4383 (PB2), pYA4384 (PB1), pYA4385 (PA), pYA4386 (NP), pYA4388 (HA), pYA4389 (NA), pYA4390 (M), pYA4391 (NS) | |
MPI-based 8-plasmid system | pYA4965 (PB1), pYA4966 (PB2), pYA4967 (PA), pYA4968 (NP), pYA4969 (HA), pYA4970 (NA), pYA4971 (M), pYA4972 (NS) | |
1-unit plasmid | pYA4973 (PB1), pYA4974 (NP), pYA4975 (NA), pYA4976 (NS); pYA4977 (PB1,GFP), pYA4978 (NP, GFP), pYA4979 (NA,GFP), pYA4980 (NS,GFP) | p15A ori, CmR |
2-unit plasmid | pYA4981 (PB1,PB2), pYA4982 (NP,PA), pYA4983 (NA,HA), pYA4984 (NS,M), pYA4985 (PB1,PB2,GFP), pYA4986 (NP,PA,GFP), pYA4987 (NS,M,GFP) | |
4-unit plasmid | pYA4988 (NP, PA, PB1, PB2,GFP), pYA4989 (NS, M, NA, HA) | |
8-unit plasmid | pYA4990 (NP, PA, PB1, PB2, NS, M, NA, HA) | |
6-unit plasmid | pYA5000 (NP, PA, PB1, PB2, NS, M) |