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Table 1 Plasmids used in the study

From: Efficient generation of influenza virus with a mouse RNA polymerase I-driven all-in-one plasmid

Plasmid type

Plasmid (related properties)

ori and marker

Vector plasmid

pcDNA3.1(−) (CMV, SV40 pA)

pUC ori, ApR

T-vector precursor

pYA4518 (AhdI-GFP cassette-AhdI)

p15A ori, CmR

Reporter plasmid

pYA4392 (CPI-EGFP), pYA4924 (MPI-EGFP)

pYA4332 (GFP cassette)

pUCori, ApR

pYA4731 (CMV-mCherry)

pYA4732 (CMV-mCherry, PB2, PB1, PA, NP, HA, NA, M, NS)

p15A ori, CmR;

MPI-based bidirectional vector

pYA4963 (CMV-MPI), pYA4964 (CMV-GFP cassette-MPI)

pUCori, ApR

CPI-based 8-plasmid system

pYA4383 (PB2), pYA4384 (PB1), pYA4385 (PA), pYA4386 (NP), pYA4388 (HA), pYA4389 (NA), pYA4390 (M), pYA4391 (NS)

MPI-based 8-plasmid system

pYA4965 (PB1), pYA4966 (PB2), pYA4967 (PA), pYA4968 (NP), pYA4969 (HA), pYA4970 (NA), pYA4971 (M), pYA4972 (NS)

1-unit plasmid

pYA4973 (PB1), pYA4974 (NP), pYA4975 (NA), pYA4976 (NS); pYA4977 (PB1,GFP), pYA4978 (NP, GFP), pYA4979 (NA,GFP), pYA4980 (NS,GFP)

p15A ori, CmR

2-unit plasmid

pYA4981 (PB1,PB2), pYA4982 (NP,PA), pYA4983 (NA,HA), pYA4984 (NS,M), pYA4985 (PB1,PB2,GFP), pYA4986 (NP,PA,GFP), pYA4987 (NS,M,GFP)


4-unit plasmid

pYA4988 (NP, PA, PB1, PB2,GFP), pYA4989 (NS, M, NA, HA)


8-unit plasmid

pYA4990 (NP, PA, PB1, PB2, NS, M, NA, HA)


6-unit plasmid

pYA5000 (NP, PA, PB1, PB2, NS, M)