Skip to main content
Fig. 2 | Virology Journal

Fig. 2

From: Gibson assembly: an easy way to clone potyviral full-length infectious cDNA clones expressing an ectopic VPg

Fig. 2

Agarose gel electrophoresis analysis of the overlapping fragments and the resulting in vitro recombination product. Analysis on a 0.8 % non-denaturing agarose gel of the three PCR amplification products in the absence of Gibson assembly mix (lane 2, negative control, incubation in water 1 h at 50 °C) and after recombination by Gibson assembly (lane 1, incubation in Gibson Assembly Master Mix 1 h at 50 °C). The black arrows indicate the position of each PCR amplification product. The red arrow indicates the position of the recombination product obtained after Gibson assembly. 1 kb+: 1 Kb Plus DNA Ladder (Invitrogen)

Back to article page