Table 3 Nucleotide sequences of the primer
From: Rescue of a duck circovirus from an infectious DNA clone in ducklings
Primersa | Sequences (5′-3′)b | Usage |
|---|---|---|
IC-1F | CCCAAGCTTGGAACTGGACCAAC | Fragment clone |
IC-1R | TCCGGATCCGAAAAATCCAAATAC | |
IC-2F | TTCGGATCCGGAACTGGACCAAC | Fragment clone |
IC-2R | TCCGAATTCGAAAAATCCAAATACGG | |
IC-MuF | GATCAGGTGACGAAGGCGCTCGAGGCCACGCCCAAAG | Genetic marker |
IC-MuR | CTTTGGGCGTGGCCTCGAGCGCCTTCGTCACCTGATC | |
PD-F | TGAACCCGGTGAACTGACC | Genetic marker detection |
PD-R | ATGCGACGCAGCACCTATC | |
SYBR-F | ACTGACGTTGCCCGGAAGTA | Quantitate virus stock |
SYBR-R | TCTTCCAATCACGTTGCGTTT |