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Figure 3 | Virology Journal

Figure 3

From: Residues 41V and/or 210D in the NP protein enhance polymerase activities and potential replication of novel influenza (H7N9) viruses at low temperature

Figure 3

Replication kinetics of recombinant viruses rgG1-WT, rgG1-NP-V41I, rgG1-NP-D210E, and rgG1-NP-V41I-D210E in MDCK cells at 33°C (A) or 37°C (B). Confluent monolayers of the various cell lines were inoculated with rgG1-WT, rgG1-NP-V41I, rgG1-NP-D210E, or rgG1-NP-V41I-D210E viruses. Cultured supernatants were harvested at 12, 24, 48, 72, and 96 hpi. Virus titers were determined by TCID50 assay using MDCK cells. Results are presented as mean ± SEM and are representative of three independent experiments. *, p < 0.05 and **, p < 0.001, compared the value of rgG1-NP-D210E with rgG1-WT.

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Virology Journal

ISSN: 1743-422X