Characterisation of secreted virus particles. A) Vero cells were infected at MOI=1 and at 18 hours post infection, were pulsed for 6.5 hours. Culture supernatant was collected and purified by immunoprecipitation using anti-E mAb (3.91D) and resolved on SDS-PAGE. B) Ratio of the integrated densities of prM to M bands indicating maturation status of secreted virus particles. C) Ratio of the integrated densities of C to E bands indicate the efficiency of nucleocapsid incorporation into virions. Bar graphs represent data from two independent experiments. Statistical analysis was performed by One-way ANOVA with multiple comparisons. ** P ≤ 0.01, *** P ≤ 0.001.