prM22/72 mutations enhanced prM/E heterodimerization. Vero cells were infected with A) unglycosylated and B) glycosylated viruses at MOI=0.1, and 18 hours later were labelled for 5 mins and chased at 0, 3, 6, and 20 mins. Metabolically-labelled prM was co-precipitated with E using anti-E antibody (3.91D), resolved by SDS-PAGE and exposed on a phosphorscreen. ImageJ analysis was performed to determine the integrated densities of bands and calculated as a prM:E ratio. Bar graphs represent data from two independent experiments. Statistical analysis was performed using t-test comparing between samples at each timepoint. * P ≤ 0.05, ns – not significant.