Figure 2

Protein expression analysis in transgenic Panax ginseng extract. (A) ELISA was used to determine the presence of antigen E^rns in extracts from transgenic ginseng hairy roots. The ELISA protocol is described in the Materials and methods section. Lane 1: recombinant proteins extracted from transgenic ginseng hairy roots. Lane 2: positive control (E^rns protein expressed in baby hamster kidney (BHK)-21 cells. Lane 3: negative control (proteins extracted from untransformed ginseng hairy roots). ^a–b The difference between the negative control (proteins extracted from untransformed ginseng hairy roots) and other groups is significant (P < 0.05). The result showed that the soluble proteins from the transgenic group had immune reactivity against anti-BVDV antiserum and the OD₄₉₀ values of the transgenic groups were significantly higher than those of the negative controls. (B) Western blot analysis showing the immune activity of E^rns protein expressed in transgenic ginseng hairy roots. In the transgenic ginseng hairy roots group (Lane 1) and the positive control [Lane 3, E^rns protein expressed in baby hamster kidney (BHK)-21 cells] a specific band of 50 kDa was detected. Lane 2, negative control (proteins extracted from untransformed ginseng hairy roots).