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Figure 1 | Virology Journal

Figure 1

From: A transgenic ginseng vaccine for bovine viral diarrhea

Figure 1

Genetic identification of transgenic ginseng hairy roots. Genomic DNA and total RNA were extracted from regenerated seedling for PCR (A) and RT-PCR (B) identification. DNA gel blot analysis was used to detect the pBI121-Erns transgene in transgenic ginseng hairy roots. (A) Lanes 3, 4, 5, and 6 show the Erns gene fragments (total genomic DNAs extracted from the leaves of different transgenic ginseng plants). Lane 1 is a negative control (total genomic DNAs extracted from the leaves of different untransformed wild-type ginseng plants). Lane 2 shows the positive control by using pBI121-Erns plasmid DNA as PCR template and lane 7 shows the molecular mass markers. (B) RT-PCR analysis. Lane 1: RT-PCR product from untransformed ginseng. Lane 2: PCR product from pBI121-Erns plasmid. Lanes 3–4: RT-PCR product from different transgenic ginseng hairy roots. Lane 5: DNA molecular mass marker.

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