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Table 3 Intra- and inter-assay coefficients of variation for multiplex rRT-PCR

From: Simultaneous detection of novel H7N9 and other influenza A viruses in poultry by multiplex real-time RT-PCR

Dilution of RNA

n

Intra-assay (%)

Inter-assay (%)

    
  

H7

N9

M

H7

N9

M

10°

6

0.97

0.96

0.91

1.48

1.38

0.14

10−2

6

0.49

1.22

0.58

0.80

0.79

0.78

10−4

6

0.38

0.32

0.11

1.01

1.34

0.98

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Virology Journal

ISSN: 1743-422X