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Figure 5 | Virology Journal

Figure 5

From: The HBx oncoprotein of hepatitis B virus potentiates cell transformation by inducing c-Myc-dependent expression of the RNA polymerase I transcription factor UBF

Figure 5

Cooperation between UBF and HBx in cell proliferation and transformation. (A) Huh7 cells transiently transfected with indicated plasmids are synchronized in S-phase by thymidine block. Following BrdU incorporation, cells were subjected to staining with antibody against BrdU and counterstained with DAPI, and the number of BrdU-positive cells was counted. Scale bar represents 50 μm. (B) IHH cells were transfected with indicated plasmids and allowed to grow in soft agar for 15 days and visualized under microscope for colony formation. Scale bar represents 10 μm. All quantitative values are mean ± S.D of three independent experiments. * represents a statistically significant difference of p <0.05.

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Virology Journal

ISSN: 1743-422X