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Figure 3 | Virology Journal

Figure 3

From: The HBx oncoprotein of hepatitis B virus potentiates cell transformation by inducing c-Myc-dependent expression of the RNA polymerase I transcription factor UBF

Figure 3

c-Myc- dependent transcriptional regulation of UBF in the presence of HBx. (A) Huh7 cells were transiently transfected with vector, HBx and/or Myc- shRNA plasmids. Total RNA was isolated and the levels of UBF mRNA were measured by RT-qPCR. (B) IHH cells were transiently transfected with vector or HBx expression plasmids. After 48 h, cells were subjected to ChIP assay using 'anti-Myc antibody. Pre-immune sera were used as a negative control. Fold DNA enrichment over mock due to UBF occupancy was measured by ChIP-qPCR using primers specific for UBF promoter (Additional file 1: Table S1). Data are shown as mean ± S.D of three independent experiments. * represents a statistically significant difference of p <0.05.

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Virology Journal

ISSN: 1743-422X