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Table 4 Reproducibility of pMDG

From: Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B

Reproducibility

No. of DNA copies

Ct (mean ± SD)

CV (%)

intra- assay

2.2 × 105

20.77 ± 0.136

0.655

2.2 × 106

16.36 ± 0.212

1.296

2.2 × 107

14.37 ± 0.042

0.292

interassay

2.2 × 105

20.34 ± 0.393

1.932

2.2 × 106

16.49 ± 0.264

1.601

2.2 × 107

14.40 ± 0.220

1.528