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Table 3 Reproducibility of pMDB β

From: Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B

Reproducibility

No. of DNA copies

Ct (mean ± SD)

CV (%)

intra- assay

3.5 × 105

17.18 ± 0.179

1.042

3.5 × 106

14.62 ± 0.066

0.451

3.5 × 107

13.59 ± 0.017

0.125

interassay

3.5 × 105

16.73 ± 0.399

2.385

3.5 × 106

14.56 ± 0.326

2.239

3.5 × 107

13.41 ± 0.185

1.380

  1. β: pMDB was obtained from CD08 cDNA with the primer of A/B-F and A/B-R.
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Virology Journal

ISSN: 1743-422X