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Table 2 Reproducibility of pMDA α

From: Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B

Reproducibility

No. of DNA copies

Ct (mean ± SD)

CV (%)

intra- assay

1.1 × 105

19.94 ± 0.191

0.958

1.1 × 106

16.38 ± 0.165

1.007

1.1 × 107

13.36 ± 0.076

0.569

interassay

1.1 × 105

20.00 ± 0.057

0.285

1.1 × 106

16.43 ± 0.133

0.809

1.1 × 107

13.76 ± 0.347

2.52

  1. α: pMDA was obtained from GD13-1 cDNA with the primer of A/B-F and A/B-R.
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Virology Journal

ISSN: 1743-422X