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Table 1 Reproducibility of pMDJ

From: Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B

Reproducibility

No. of DNA copies

Ct (mean ± SD)

CV (%)

intra- assay

5.5 × 105

18.56 ± 0.147

0.792

5.5 × 106

15.32 ± 0.016

0.104

5.5 × 107

13.90 ± 0.139

1.000

interassay

5.5 × 105

18.81 ± 0.233

1.239

5.5 × 106

15.05 ± 0.275

1.827

5.5 × 107

13.82 ± 0.081

0.586