Figure 2

Kinetics of mRNA expression of receptors (TLR-3, TLR-7, TLR-8, RIG- I and MDA5) ( A , B ), signaling proteins (MyD88, MAVS, TRIF, TRAF-3, IRF-3, and IRF-7) ( C ) and effector molecules (TNF-α, IL-1β, IFN-α and IFN-β) ( D ) after virus infection. PAMs were infected with SIV (A/Swine/Shandong/3/2005) at a multiplicity of infection of 5. Mock-treated cells received virus-free culture medium. Macrophages were harvested for RNA isolation at 0, 2, 4, 6, 8, 12, and 24 hpi. Data are presented as means ± SEM (n = 3). Results are representative of 3 independent experiments.