Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis

Wang, Li; Xu, Xiaoxing; Zhang, Huiping; Qian, Jihong; Zhu, Jianxing

doi:10.1186/s12985-015-0281-9

Virology Journal

Table 3 Characteristics of the included studies

From: Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis

Author [Reference]	Year	Location	Design	Total population screened	Inclusion criteria	Size of DBS used (diameter)	DBS stored temperature	Time of samples tested to collected	Type of DNA extraction	Type of PCR	Target region	Gold standard
Barbi et al [10]	1998	Italy	Prospective	205	Universal screening	3 disks (3mm)	UN	UN	Heat shock	Nested-PCR	UL55	Viral isolation from saliva
Barbi et al [8]	2006	Italy	Retrospective	874	Suspected of cCMV infection	3 disks (3mm)	UN	UN	Heat shock	Real-time PCR	gB	Viral isolation from urine or saliva
Binda et al [11]	2004	Italy	Retrospective	195	Suspected of cCMV infection	3 disks (3mm)	UN	UN	modified Heat shock	Nested-PCR	gp58	Viral isolation from urine,saliva or blood
Boppana et al [2]	2010	America	Prospective	11407	Universal screening	2 disks (3mm)	room temperature	14.6±9.6days	Qiagen M48 robotic system	Single-primer Real-time PCR	gB	The DEAFF assay on the follow-up saliva/urine sample
Boppana et al [2]	2010	America	Prospective	9018	Universal screening	2 disks (3mm)	room temperature	14.6±9.6days	Qiagen M48 robotic system	Two-primer Real-time PCR	gB & IE2	The DEAFF assay on the follow-up saliva/urine sample
Distéfano et al [24]	2008	Argentina	Retrospective	145	Compatible symptoms	UN	UN	UN	Heat shock	Nested-PCR	gB	Viral isolation from urine
Johansson et al [22]	1997	Sweden	Retrospective	31	infants confirmed with/without cCMV and infants whose samples were stored close to the infetive ones	25mm2	4°C	12-18 years	Phenol-chloroform	PCR+hybridization test	OP1, OP2, IE1	Viral isolation from urine
Leruez-Ville et al [12]	2009	France	Retrospective	214	Compatible symptoms, Maternal PI	UN	UN	UN	QiAamp DNA Blood Mini kit (Qiagen)	Real-time PCR	UL123exon 4	Urine culture/PCR
Leruez-Ville et al [26]	2011	France	Prospective	271	Compatible symptoms, Maternal PI	whole spot (10mm)	standard conditions	After metabolic screening	QiAamp DNA Blood Mini kit (Qiagen)	Real-time PCR	UL123exon 4	Urine sample PCR
Paixão et al [25]	2009	Portuguese	Retrospective	308	Neonates confirmed with/without cCMV infection	UN	UN	UN	Heat shock	Nested-PCR	gp58	Urine shell-viral culture
Paradizˇ et al [13]	2012	Slovenia	Prospective	2841	Universal screening	8 discs (2mm)	room temperature	7 days	QiAamp DNA Blood Micro kit (Qiagen)	Real-time PCR	UN	Urine sample PCR
Scanga et al [14]	2006	America	Retrospective	19	infants confirmed with/without cCMV and infants whose samples were stored close to the infetive ones	whole spot (10mm)	room temperature	2-20 months	QiAamp DNA Blood Micro kit (Qiagen)	Real-time PCR	POL	Urine culture
Soetens et al [16]	2008	Brussels	Retrospective	67	Universal screening	whole spot (10mm)	room temperature	72.9±31 months (0.5-130 months)	Phenol chloroform	Conventional PCR + nested-PCR	US8 & gH	Urine culture
Vaudry et al [15]	2010	Canada	Prospective	95	infants with VLBWs or SGA	UN	UN	about one month	MagaZorb DNA extraction Kit (Cortex Biochem)	CMV LC-real time PCR (Roche Diagnostic)	gB1, gB2	Viral isolation from throat swabs
Yamamoto et al [21]	2001	Brazil	Prospective	332	Universal screening	3 disks (6mm)	-20°C	UN	Heat shock	Nested-PCR	MIE & gB/gB1 & IE	Urine sample viral culture and/or PCR

We extracted characteristics of the 15 studies, regarding to sample group information, DBS sample collection, DNA extraction and PCR methods. In Boppana et al. [2] study, they enrolled two groups of subjects and used different methods, therefore this article was deemed to be two independent studies. In Distéfano et al [24] study, 15 of 145 cases were identified as perinatal CMV infection, they were not included in this meta-analysis.

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ISSN: 1743-422X

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