Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis

Table 1 The calculation methods of LOD

Author,Year	Blood sample volume V1	Dilution volume V2	Elute used in PCR assay volume V3	Whole blood volume in each PCR reaction V4	LOD1 copies/PCR reaction	LOD2 copies/ml whole blood
Barbi et al [10]	50	25	2	4	2	500
Binda et al [11]	40	45	10	8.89	4	449.9
Boppana et al [2]	8	30^a	5	1.3	1.56	1200^d
Leruez-Ville et al [12]	50	50	10	10	40	4000
Paradizˇ et al [13]	16	100	20	3.2	4.15	1296
Scanga et al [14]	50	100	10	5	8	1600
Soetens et al [16]	50	25	5^c	10	94	9400
Vaudry et al [15]	50	50^b	5	5	8	1600

a. The volume is recommended in the DNA extraction kit (Qiagen) used in the study, may not the true volume in the tests. b. The volume is recommended in the DNA extraction kit (Magazorb, USA) used in the study, may not the true volume in the tests. c. The data was referenced study by Leruez-Ville et al [36]. d. We chose the minimum dilution in the sensitivity titration assays to evaluate the LOD, because the data described in the article seems not reliability.
\( \mathrm{L}\mathrm{O}\mathrm{D}1=\frac{LOD2\times {\mathrm{V}}_4}{1000}=\frac{LOD2\times {V}_3\times {V}_1}{1000\times {V}_2} \) (Assuming a 100% yield of extraction).

ISSN: 1743-422X