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Table 1 p27 antigen of ALV-J shedding tested by ELISA

From: Synergistic inhibition of avian leukosis virus subgroup J replication by miRNA-embedded siRNA interference of double-target

 

2d

4d

6d

8d

10d

12d

16d

20d

22d

24d

28d

normal control

0.04 ± 0.08

0.04 ± 0.17

0.02 ± 0.06

0.11 ± 0.10

0.03 ± 0.10

0.01 ± 0.06

0.02 ± 0.07

0.01 ± 0.01

0.07 ± 0.05

0.04 ± 0.04

0.07 ± 0.04

null control

0.26 ± 0.02

1.08 ± 0.08

0.46 ± 0.22

1.14 ± 0.21

1.25 ± 0.55

0.43 ± 0.06

1.73 ± 0.76

0.44 ± 0.21

0.86 ± 0.21

0.47 ± 0.24

1.33 ± 0.19

ALV-J control

0.57 ± 0.08

1.23 ± 0.05

0.99 ± 0.37

0.29 ± 0.12

1.43 ± 0.24

0.24 ± 0.09

1.12 ± 0.18

0.45 ± 0.37

1.26 ± 0.08

0.24 ± 0.11

1.36 ± 0.30

p-miR-gag + LTR

0.06 ± 0.08

0.09 ± 0.10

0.08 ± 0.12

0.05 ± 0.06

0.03 ± 0.07

0.08 ± 0.07

0.26 ± 0.10a

0.03 ± 0.07

0.05 ± 0.03

0.31 ± 0.17

0.43 ± 0.15

p-miR-pol + LTR

0.03 ± 0.01

0.02 ± 0.00

0.02 ± 0.00

0.03 ± 0.00

0.01 ± 0.00

0.04 ± 0.02

0.03 ± 0.08

0.02 ± 0.05

0.21 ± 0.08a

0.38 ± 0.06

0.19 ± 0.10

p-miR-env + LTR

0.03 ± 0.00

0.02 ± 0.00

0.02 ± 0.01

0.13 ± 0.00

0.02 ± 0.00

0.04 ± 0.03

0.14 ± 0.01

0.02 ± 0.02

0.33 ± 0.04a

0.37 ± 0.17

0.08 ± 0.13

p-miR-gag

0.04 ± 0.00

0.02 ± 0.00

0.06 ± 0.00

0.08 ± 0.01

0.17 ± 0.00

0.31 ± 0.04a

0.02 ± 0.07

0.22 ± 0.06

0.3 ± 0.08

0.02 ± 0.06

0.38 ± 0.23

p-miR-pol

0.05 ± 0.01

0.08 ± 0.01

0.12 ± 0.00

0.20 ± 0.00a

0.25 ± 0.01

0.02 ± 0.03

0.09 ± 0.01

0.01 ± 0.05

0.31 ± 0.13

0.22 ± 0.14

0.43 ± 0.15

p-miR-env

0.01 ± 0.01

0.01 ± 0.01

0.02 ± 0.00

0.01 ± 0.01

0.02 ± 0.00

0.15 ± 0.11

0.22 ± 0.06a

0.01 ± 0.05

0.01 ± 0.15

0.04 ± 0.05

0.32 ± 0.07

p-miR-LTR

0.04 ± 0.01

0.03 ± 0.01

0.04 ± 0.04

0.18 ± 0.01

0.29 ± 0.00a

0.22 ± 0.03

0.01 ± 0.06

0.11 ± 0.21

0.44 ± 0.06

0.32 ± 0.18

0.09 ± 0.11

  1. Note: aindicate the first time of shedding p27 antigen of ALV-J. Cut off value = 0.2.