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Figure 3 | Virology Journal

Figure 3

From: Synergistic inhibition of avian leukosis virus subgroup J replication by miRNA-embedded siRNA interference of double-target

Figure 3

Single target and double target interference efficiency detected by IFA. The 200 ng single target miRNA-embedded siRNA expression plasmids and p-miR-LTR (100 ng) combination with p-miR-gag (100 ng), p-miR-pol (100 ng) and p-miR-env (100 ng) were transfected with ALV-J into DF-1 cells and gp85 protein of ALV-J expression was monitored 72 h post-transfection by fluorescence microscopy (magnification 40×). The primary antibody is anti-gp85 monoclonal antibody (1D4), and the second antibody is PE-labeled anti-mouse antibody.

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Virology Journal

ISSN: 1743-422X