Figure 2
From: Clinical response to pandemic h1n1 influenza virus from a fatal and mild case in ferrets
Lung histopathology and NP inmunohistochemestry of ferrets with different clinical symptoms. (A) Histopathology of a representative control ferret. Hematoxilin/Eosin (HE) stain (20x objective field); (B) Histopathology of a representative ferret which presented NS signs and bronchopneumonia. Bronchiolar-alveolar junction showing mild lymphoplasmacytic infiltration and bronchial epithelium slough. HE stain (20x objective field; (C) Histopathology of a representative ferret presenting S signs exhibiting severe clinical signs and interstitial pneumonia. Detail of the alveolar interstitium showing diffuse alveolar damage with presence of hyaline membranes, edema, large macrophagic infiltrate and hemorrhage. HE stain (20x objective field); (D) NP staining in a representative control ferret. Haematoxylin counterstain (40x objective field); (E) NP staining in a representative ferret belonging to the NS group showing viral antigen present only in bronchial epithelia. Haematoxylin counterstain (40x objective field); (F) NP staining in a representative ferret belonging to the S group, showing viral antigen staining in pneumocytes and alveolar macrophages. Haematoxylin counterstain (40x objective field).