Effects of BiP silencing on persistently JEV-infected cell clones. (A) Persistently JEV-infected cell clones, cBS6-2 and cBS6-3, were transfected with specific siRNA for BiP or non-specific scramble siRNA at the indicated concentration for 40 hr. The cell lysates were collected to measure the expression levels of BiP, CHOP, caspase-3, JEV NS3, and β-actin as an internal control. (B) The number of viable cells from the sample of (A) was determined using trypan blue exclusion. *P < 0.05.