Effects of CHOP silencing on wild-type JEV infection. (A) BHK-21 cells were transfected with specific siRNA for CHOP or non-specific scramble siRNA and infected with JEV at an MOI of 1 or mock infected at 24 hr post-transfection. Cells were harvested at 48 hr p.i. The cell lysates were subjected to Western blotting with CHOP, Bcl-2, caspase-3, JEV NS3, and β-actin as an internal control. The number of viable cells (B) and infectious virus particles (C) from the (A) sample were determined by trypan blue exclusion and plaque-forming assay, respectively. *P < 0.05, **P < 0.005.