Figure 4

Formononetin could inhibit EV71-induced COX-2 expression and PGE ₂ production. (A) Formononetin but not pirodavir can reduce the expression of COX-2 induced by EV71 infection. SK-N-SH cells were mock-infected or infected with 480 pfu/well of EV71 (SHZH98) for 2 h. The cells were then treated with formononetin (14.91 μmol/L) and pirodavir (1.08 μmol/L), respectively, for 24 h. The cells were harvested and EV71-VP1 and COX-2 were examined by western blot. (B) Formononetin but not pirodavir can reduce the release of PGE₂ stimulated by EV71 infection. SK-N-SH cells were pretreated with formononetin (14.91 μmol/L) or pirodavir (1.08 μmol/L) for 24 h. The cells were infected with 4.8 × 10⁵ pfu/well of EV71 (SHZH98). At 24 h p.i., the culture supernatants were harvested for PGE₂ release assay.