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Table 4 Evaluation of the response of transgenic tomato lines harbouring the hpRNAi construct to challenge with TYLCV-OM and TYLCV-OM/ToLCB-OM

From: RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite

Transgenic line

TYLCV-OM (30 dpi)

TYLCV-OM/ToLCB (30 dpi)

TYLCV-OM (60 dpi)

TYLCV-OM/ToLCB (60 dpi)

 

Plants symptomatic/inoculated

SS*

% Resistance

Plants symptomatic/inoculated

SS*

% Resistance

Plants symptomatic/inoculated

SS*

% Resistance

Plants symptomatic/inoculated

SS*

% Resistance

11

2/20

1

90

4/20

1

80

5/20

1

75

6/20

1

70

12

2/20

1

90

4/20

1

80

4/10

2

80

7/20

2

65

13

1/20

1

95

6/20

1

70

1/20

1

95

7/20

1

65

18

2/20

1

90

4/20

2

80

5/20

2

75

7/20

2

65

21

0/20

0

100

5/20

1

75

4/20

1

80

7/20

1

65

23

1/20

1

95

2/20

1

90

5/20

1

75

9/20

1

55

41

0/20

0

100

4/20

1

80

0/20

0

100

4/20

1

80

51

2/20

1

90

4/20

2

80

4/20

1

80

7/20

1

65

52

0/20

0

100

2/20

1

90

4/20

1

80

5/20

1

75

NC**

0/10

-

-

0/10

-

-

0/10

-

-

0/10

-

-

PC***

10/10

1

0

10/10

2

0

10/10

3

0

10/10

3

0

  1. The data presented in each case is the sum of two independent experiments with 10 plants each.
  2. *Symptom severity rated according to AVDRC disease severity scale (0: Normal healthy plant, 1: light leaf yellowing, 2: moderate plant stunting with leaf yellowing and curling, 3: Severe plant stunting with leaf curling and yellowing).
  3. **Non-transgenic tomato cv. Pusa Ruby plants inoculated with pGreen0029 vector with no insert.
  4. ***Non-transgenic tomato cv. Pusa Ruby plants inoculated with TYLCV-OM or TYLCV-OM/ToLCB.