RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite

Table 1 Effect of the transient expression of the hpRNAi construct on the infectivity of TYLCV-OM and TYLCV-OM/ToLCB-OM in Nicotianabenthamiana plants

Inoculum	Experiment	Infectivity (plants symptomatic/inoculated)			SS*	% Resistance	Molecular diagnosis
		15 dpi	30dpi	40 dpi			PCR	Southern for TYLCV-OM	Southern for ToLCB-OM
Non-inoculated	-	0/10	0/10	0/10	0	NA	_	_	_
TYLCV-OM	I	10/10	10/10	10/10	3	-	10/10	10/10	_
	II	10/10	10/10	10/10	3	-	10/10	10/10	_
	III	10/10	10/10	10/10	3	-	10/10	10/10	_
TYLCV-OM/ ToLCB-OM	I	10/10	10/10	10/10	3	-	10/10	10/10	10/10
	II	10/10	10/10	10/10	3	-	10/10	10/10	10/10
	III	10/10	10/10	10/10	3	-	10/10	10/10	10/10
TYLCV-OM + hpRNAi	I	0/10	1/10	2/10	1	80	6/10	0/10	0/10
	II	0/10	2/10	2/10	1	80	4/10	0/10	0/10
	III	0/10	3/10	4/10	2	60	6/10	1/10	0/10
TYLCV-OM/ToLCB-OM + hpRNAi	I	0/10	3/10	4/10	1	60	7/10	0/10	0/10
	II	0/10	3/10	3/10	2	70	6/10	3/10	2/10
	III	0/10	2/10	3/10	2	70	8/10	4/10	4/10

*Symptom severity for symptomatic plants rated according to AVDRC disease severity scale (0: Normal healthy plant, 1: light leaf yellowing, 2: moderate plant stunting with leaf yellowing and curling, 3: Severe plant stunting with leaf curling and yellowing).

ISSN: 1743-422X