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Figure 5 | Virology Journal

Figure 5

From: RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite

Figure 5

Real-time quantitative PCR assay of TYLCV-OM in transgenic and non-transgenic tomato cv. Pusa Ruby plants inoculated with TYLCV-OM and TYLCV-OM/ToLCB-OM. The bars indicate calculated virus titre (genome copies per 25 ng total DNA) in each case. The results are from qPCR reactions with DNA extracted from inoculated non-transgenic tomato cv. Pusa Ruby plants (C), a non-inoculated non-transgenic plant (N) and four plants each of 9 transgenic Pusa Ruby lines (as indicated on the figure) that were inoculated. For all the inoculated plants the left two bars (indicated by a yellow block at the base) are plants inoculated with only TYLCV-OM and the right two bars (indicated by a green block at the base) are plants inoculated with TYLCV-OM and ToLCB-OM. Each bar is the mean of three replicates of the qPCR reaction and the error bars indicate standard deviation. The leaf samples from which DNA was extracted were collected at 60 dpi.

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Virology Journal

ISSN: 1743-422X