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Figure 4 | Virology Journal

Figure 4

From: RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite

Figure 4

Southern blot analysis of transgenic tomato plants harboring the hpRNAi construct inoculated with TYLCV-OM probed for the presence of TYLCV-OM (A) and inoculated with TYLCV-OM/ToLCB-OM probed for the presence of ToLCB-OM (B). The DNA samples run on the gel were extracted from a non-transgenic non-inoculated tomato cv. Pusa Ruby plant (N), symptomatic TYLCV-OM (panel A) or TYLCV-OM/ToLCB-OM (panel B) inoculated non-transgenic Pusa Ruby plants (C) and two plants each of 9 transgenic Pusa Ruby lines (as indicated on the figure) inoculated with either TYLCV-OM (panel A) or TYLCV-OM/ToLCB-OM (panel B). Approx. 10 μg of total DNA was loaded on each case and the samples were extracted at 60 dpi. The positions of the viral single-stranded (ss) and supercoiled (sc) replicative DNA forms are indicated.

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Virology Journal

ISSN: 1743-422X