Detection of EBV expression in the spleen and liver of EBV infected rabbits. All EBER-positive spleens were tested for the expression of EBV LMP-1 by immunohistochemistry (A). Characteristic membrane and cytoplasmic staining was evident in the spleen of all 3 rabbits (A) bottom panel. Formalin-fixed and paraffin-embedded EBV-positive cell line B95-8 was used as positive control. Expression of EBNA-1 and EBNA-2 was examined by western blotting (B). A total of 50 μg of protein lysate was loaded per well. Specific bands were noted in both the liver (L) and spleen (S) of infected animals. Protein extracts from B95-8 cells was used as positive control.