Comparison of the distributed threshold cycle (Ct) values. The Ct values of samples identified as positive by both GeneXpert Enterovirus Assay (GXEA) and real-time one-step RT-PCR (RTo-PCR) (GXEA(+)/TaqMan(+):●) and the Ct values of GXEA positive but RTo-PCR assay negative (GXEA(+)/TaqMan(+):■) samples were graphed, respectively. The Ct mean value and standard deviation (S.D.) of double-positive samples was 32.38 and 2.48 respectively. The Ct mean value and S.D. of GXEA positive but RTo-PCR negative samples was 34.85 and 2.18 respectively.