Table 2 Primers and probes used for the duplex real-time PCR assay
Primer/probe name | Primer/probe sequence | Target gene | Amplicon length |
|---|---|---|---|
ARV F | 5’-CGTTCCCTGTGGACGTATCA-3’ | σC | 69 bp |
ARV R | 5’-GAGTACACCCCATACGCTTGGT-3’ | ||
ARV P | 5’-(FAM) TCACCCGCGATTCTGCGACTCAT (Eclipse)-3’ | ||
MS-F | 5’-ATAGCAATTTCATGTGGTGATCAA-3’ | vlhA | 143 bp |
MS-R | 5’-TGGATTTGGGTTTTGAGGATTA-3’ | ||
MS-P | 5’-(ROX) CAGCACCTGAACCAACACCTGGAA (Eclipse)-3’ |