Mode of inhibition of DENV-2 NS2b-NS3Pro by MB21. (A) Enzymatic activity of DENV-2 NS2b-NS3Pro as a function of substrate concentration in the absence (filled circles) and presence of 6 μM MB21 (empty circles). The table shows kinetic parameters in presence (E + S + I) and absence (E + S) of MB21. E, S and I denote the enzyme, DENV-2 NS2b-NS3Pro, the substrate, Bz-nKRR-AMC, and the inhibitor, MB21. (B) Lineweaver-Burke plot of DENV-2 NS2b-NS3Pro activity in the absence (filled circles) and presence of 6 μM MB21 (empty circles). Data were analyzed using GraphPad Prism software.