Figure 4From: Inhibition of IRS-1 by hepatitis C virus infection leads to insulin resistance in a PTEN-dependent manner Effect of transfection of Huh7.5.1 cells with JFH1-core plasmid on PTEN expression. Huh7.5.1 cells transfected with empty vector or core expression vector were subjected to western blotting for pIRS1Ser307, IRS-1, PTEN and HCV core-specific antibodies. GAPDH was used as an internal control to verify protein loading in each well.Back to article page