Figure 3
Effect of HCV infection on PTEN expression in Huh7.5.1 cells. Huh7.5.1 cells were routinely infected with different M.O.I of HCV 2a cc for 48 h and equal amounts of cellular lysates were subjected to western blotting for evaluating PTEN protein level (a) and qRT-PCR for PTEN mRNA expression (b). Huh7.5.1 cells, empty lentivector groups, and PTEN or PTEN shRNA expressing cells were infected with HCV viral particles (M.O.I = 2). Representative immunoblots of pIRS-1Ser307, IRS-1, PTEN and GAPDH expression in cells showing PTEN overexpression (c) and downregulation (d), respectively.