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Table 1 RealTime results and Ct values from DNA extracted from ICC and CIN2/3 specimens using heat or xylene treatment

From: Identification of human papillomaviruses from formalin-fixed, paraffin-embedded pre-cancer and invasive cervical cancer specimens in Zambia: a cross-sectional study

 

Heat

Xylene

 

RealTime result

n

n

 

  Invalida

2

2

 

  No HPV detected

2

4

 

  Single HPV infection

18

19

 

  Dual HPV infections

4

2

 

  Triple HPV infections

1

0

 

  Total

27

27

 

Abbott Fluorophore/channel

n

Mean Ct value

n

Mean Ct value

p value b

   β-globin internal control

25

27.7

24

30.6

<0.001

  HPV-16

14

23.8

12

25.3

0.349

  HPV-18

3

26.3

3

29.9

0.222

  other HR HPV

13

28.1

9

31.0

0.156

  1. a β-globin internal control was either negative or had a Ct count >35.
  2. bStudent’s t-test.
  3. Results represent three separate experiments of 9 specimens each (27 FFPE specimens total; 17 ICC and 10 CIN 2/3). Ct values above 35 were excluded.
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Virology Journal

ISSN: 1743-422X