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Table 1 RealTime results and Ct values from DNA extracted from ICC and CIN2/3 specimens using heat or xylene treatment

From: Identification of human papillomaviruses from formalin-fixed, paraffin-embedded pre-cancer and invasive cervical cancer specimens in Zambia: a cross-sectional study

  Heat Xylene  
RealTime result n n  
  Invalida 2 2  
  No HPV detected 2 4  
  Single HPV infection 18 19  
  Dual HPV infections 4 2  
  Triple HPV infections 1 0  
  Total 27 27  
Abbott Fluorophore/channel n Mean Ct value n Mean Ct value p value b
   β-globin internal control 25 27.7 24 30.6 <0.001
  HPV-16 14 23.8 12 25.3 0.349
  HPV-18 3 26.3 3 29.9 0.222
  other HR HPV 13 28.1 9 31.0 0.156
  1. a β-globin internal control was either negative or had a Ct count >35.
  2. bStudent’s t-test.
  3. Results represent three separate experiments of 9 specimens each (27 FFPE specimens total; 17 ICC and 10 CIN 2/3). Ct values above 35 were excluded.