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Table 1 Primers used for the generation of E1/E2 area of HCV genotype 4

From: New insight into HCV E1/E2 region of genotype 4a

Primers

Polarity

Sequences (5′-3′)

Application

Product size

HCV outer 2

Antisense

CACCAGCGGGTGAAGCAGCATTGA

RT/1st round PCR

 

HCV outer 1

Sense

GGACGGGGTAAACTATGCAACAGG

1st round PCR

1804 bp

HCV inner 2

Antisense

GACAGTTACGCCTGAACTTGACTTACCATAAACATC

2nd round PCR

 

HCV inner 1

Sense

CACCCATGGGTTGCTCTTTTTCTATC

2nd round PCR

1726 bp