Table 1 Primers used for the generation of E1/E2 area of HCV genotype 4
| Primers | Polarity | Sequences (5′-3′) | Application | Product size |
|---|---|---|---|---|
| HCV outer 2 | Antisense | CACCAGCGGGTGAAGCAGCATTGA | RT/1st round PCR | |
| HCV outer 1 | Sense | GGACGGGGTAAACTATGCAACAGG | 1st round PCR | 1804 bp |
| HCV inner 2 | Antisense | GACAGTTACGCCTGAACTTGACTTACCATAAACATC | 2nd round PCR | |
| HCV inner 1 | Sense | CACCCATGGGTTGCTCTTTTTCTATC | 2nd round PCR | 1726 bp |