Figure 6

EV71 infection stimulates the expression of SOS1 protein. EV71 infection: EV71-infected iDCs. EV71 infection + U0126: EV71-infected iDCs in the presence of U0126 (20 μM). iDCs were infected with EV71 at a MOI of 5. Cells were collected and lysed at 0 h, 1/2 h, 2 h, 4 h, 8 h and 24 h p.i. SOS1 expression at different time points were analyzed by western blot. iDCs were pre-incubated with U0126 (20 μM) for 1 h, and then infected with EV71 at a MOI of 5. The intensity of SOS1 protein band was quantitated by densitometric analysis and normalized to GAPDH. The data were expressed as mean ± SE from three independent experiments and analyzed by one-way or two-way ANOVA (**p < 0.01, ***p < 0.001).