Figure 5

The phosphorylation of nuclear transcription factor c-myc and Elk1. iDCs were infected with EV71 at a MOI of 5. Cells were collected and lysed at 0 h, 1/2 h, 2 h, 4 h, 8 h and 24 h p.i., and GAPDH was used as an internal control. The total or phosphorylated c-myc and Elk1 at different time points were analyzed by western blot (A and C). iDCs were pre-incubated with U0126 (20 μM) for 1 h, and then infected with EV71 at a MOI of 5 (B and D). The ratios of p-c-myc to c-myc and p-Elk1 to Elk1 at different time were analyzed by densitometric scanning. The data were expressed as mean ± SE from three independent experiments and analyzed by one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001).