Figure 4

Activation of c-Fos and c-Jun by EV71 infection. iDCs were infected with EV71 at a MOI of 5. Cells were collected and lysed at 0 h, 1/2 h, 2 h, 4 h, 8 h and 24 h p.i., and GAPDH was used as an internal control. The total or phosphorylated c-Fos and c-Jun at different time points were analyzed by western blot (A and C). iDCs were pre-incubated with U0126 (20 μM) for 1 h, and then infected with EV71 at an MOI of 5 (B and D). The ratios of p-c-Fos to c-Fos and p-c-Jun to c-Jun at different times were analyzed by densitometric scanning. The data were expressed as mean ± SE from three independent experiments and analyzed by one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001).