Figure 3

Activation of MEK1/2 and ERK1/2 by EV71 infection and its inhibition by U0126. iDCs were infected with EV71 at a MOI of 5. Cells were collected at 0 h, 1/2 h, 2 h, 4 h, 8 h and 24 h p.i., and lysed for western blot analysis. GAPDH was used as an internal control. The total or phosphorylated MEK1/2 and ERK1/2 at different time points were analyzed by western blot (A and C). iDCs were pre-incubated with U0126 (20 μM) for 1 h, and then infected with EV71 at a MOI of 5, U0126 was present throughout the experiment (B and D). The ratios of p-MEK1/2 to MEK1/2 and p-ERK1/2 to ERK1/2 at different times were analyzed by densitometric scanning. The data were expressed as mean ± SE from three independent experiments and analyzed by one-way ANOVA (*p < 0.05, **p < 0.01,***p < 0.001).