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Figure 2 | Virology Journal

Figure 2

From: MEK/ERK signaling pathway is required for enterovirus 71 replication in immature dendritic cells

Figure 2

The inhibitory effects of U0126 on EV71 replication. (A) iDCs (3 × 105/well) pretreated with or without U0126 (20 μM) for 1 h and infected with EV71 (MOI = 5) for 24 h. Culture supernatants were collected after infection to determine viral titers. (B) Western blot results of the supernatants and cell lysates of iDCs pre-incubated without or with U0126 (20 μM) for 1 h and infected with EV71 at a MOI of 5, using a specific antibody against VP1. The intensity of VP1 protein band was quantitated by densitometric analysis and normalized to GAPDH. The data were expressed as mean ± SE from three independent experiments and analyzed by two-way ANOVA with Bonferroni post-hoctests (**p < 0.01,***p < 0.001).

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