Figure 1

Flow cytometric analysis of human blood monocyte-derived iDCs and EV71-infected iDCs. (A) Monocytes cultured in the medium containing GM-CSF and IL-4 for 7 days were analyzed by flow cytometry with isotype-matched control (thin lines) using specific antibodies (red lines). The data shown are representative of three independent experiments. (B) Uninfected iDCs (control) or iDCs infected with EV71 (MOI = 5) were analyzed using flow cytometry for the expression of CD80, CD83, CD86 and HLA-DR at 24 h p.i. using isotype-matched control (thin lines) and specific antibodies (red lines). The data were expressed as mean ± SE from three independent experiments and analyzed by Chi-square test.