Figure 4
Effect of low expression level of Nef mac239 on SIV mac239 infectivity. pBRmac239Δnef/luc was cotransfected into HEK293 cells with 1 μg of a mixture of pNefmac239-WT and an empty vector, at amounts of pNefmac239-WT in grams indicated. After a 72 h cultivation, the cells were collected and subjected to SDS-PAGE and western immunoblot analysis to detect Nef and β-actin, as described in Materials and Methods (A). The supernatants were subjected to SIV p27 ELISA and infectivity assay using MAGIC-5 cells as described in Materials and Methods (B). Each bar represents the mean standard deviation (n = 3).