Effect of hnRNP C1/C2 knockdown on release of infectious DENV. Huh7 cells transfected with hnRNP C1/C2-specific siRNA or control siRNA were infected with DENV at MOI 0.03. Supernatant from DENV-infected culture was collected at 0, 12, 24, 36 and 48 h post-infection and assessed for titers of infectious DENV by FFU assay. Results are derived from three independent experiments. Asterisks indicate statistically significant differences (**p < 0.01; ***p < 0.005) in DENV titers between the specific siRNA-transfected and control siRNA-transfected groups by unpaired t test.