Figure 5

Effect of hnRNP C1/C2 knockdown on the extent of DENV infection. Huh7 cells transfected with hnRNP C1/C2-specific siRNA or control siRNA were infected with DENV at MOI 0.03 or left uninfected (mock control). The cells were collected at 12, 24, 36 and 48 h post-infection and subjected to immunofluorescence staining for DENV E antigen. The extent of DENV infection was determined based on the enumerated numbers of DENV E antigen-positive cells. (A) Representative images of immunofluorescently stained cells from three independent experiments. Images of mock-infected cells at 24 h post-infection and DENV-infected cells at 12, 24, 36 and 48 h post-infection are shown. Dengue E antigen (green); nucleus (blue); magnification (63×). (B) Percentage infection is shown as mean and SEM of three independent experiments. Asterisks indicate statistically significant differences (**p < 0.01) in the percentage DENV infection between the specific siRNA-transfected and control siRNA-transfected groups by unpaired t test.