Effect of hnRNP C1/C2 knockdown on DENV RNA synthesis. Huh7 cells transfected with hnRNP C1/C2-specific or control siRNA were infected with DENV at MOI 0.03. Cells were collected at 0, 12, 24, 36 and 48 h post-infection and subjected to total RNA extraction and subsequent reverse transcription and real-time PCR for determination of DENV RNA expression. The amount of DENV RNA in virus-infected cells was reported as viral RNA copies per cell. Data represent mean and SEM of three independent experiments. Asterisks indicate statistically significant differences (*p < 0.05) in DENV viral RNA levels between the specific siRNA-transfected and control siRNA-transfected groups by unpaired t test.