Association of hnRNP C1/C2 proteins with dengue viral RNA. Mock-infected (M) and DENV-infected (I) cells were subjected to immunoprecipitation using anti-hnRNPC1/C2 (αhnRNP C) monoclonal antibody and their isotype-matched control antibody (IgG1). (A) Immunoprecipitated proteins were analyzed by immunoblotting using hnRNP C1/C2-specific antibody. Mock and DENV-infected cell lysates prior to immunoprecipitation served as controls (input). (B) RNA was extracted from the immunoprecipitated samples and used as a template for RT-PCR with a primer pair specific for DENV NS1 region. PCRs performed in parallel in the absence of cDNA and in the presence of pcDNAhygro containing DENV NS1 gene were included as negative (−) and positive (+) controls, respectively. Results are representative of three independent experiments with similar outcome.