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Figure 3 | Virology Journal

Figure 3

From: Kaposi’s sarcoma-associated herpesvirus G-protein coupled receptor activates the canonical Wnt/β-catenin signaling pathway

Figure 3

Effect of β-catenin knockdown on tubule formation and β-catenin target gene expression. HUVECs expressing vGPCR were transiently transfected with siRNA to β-catenin or with non-silencing siRNA control. (A) 72 hr following β-catenin silencing, knockdown efficiency was determined by qRT-PCR. β-catenin mRNA levels were normalized to 36B4 expression. (B) vGPCR-HUVECs were plated on Matrigel as described in Materials and Methods and allowed to form tubules. (C) The number of branch points was determined 6 hr after plating. The data is presented as an average of two independent experiments performed in duplicate. ***p < 0.001.

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