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Table 2 Primers for PCR and RT-PCR amplification of the different PERV subtypes, mtDNA , pocine β-actin and human GAPDH

From: Characterization of PERV in a new conserved pig herd as potential donor animals for xenotransplantation in China

Gene Primer sequence Fragment size (bp)
PERV-A F: 5′-TGGAAAGATTGGCAACAGCG-3′ 360
R: 5′-AGTGATGTTAGGCTCAGTGG-3′
PERV-B F: 5′-TTCTCCTTTGTCAATTCCGG-3′ 264
R: 5′-TACTTTATCGGGTCCCACTG-3′
PERV-C F: 5′-CTGACCTGGATTAGAACTGG-3′ 281
R: 5′-ATGTTAGAGGATGGTCCTGG-3′
pol F: 5′-CCACAGGGCAACGGCAGTATCC-3′ 212
R: 5′-TTGGAGGGTCAACACAGTGATGG-3′
gag F: 5′-CGGCAAGAGAAGAATTTGACT-3′ 188
R: 5′-CAGTTCCTTGCCCAGTGTCC-3′
mtDNA F: 5′-TCACCCATCATAGAAGAACTCCTACA-3′ 281
R: 5′-TTTTACGGTTAAGGCTGGGTTATTAAT-3′
β-actin F: 5′-CACGCCATCCTGCGTCTGGA-3′ 100
R: 5′-AGCACCGTGTTGGCGTAGAG-3′
GAPDH F: 5′-CAAGGTCATCCATGACAACTTTG-3′ 496
R: 5′-GTCCACCACCCTGTTGCTGTAG-3′