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Table 2 Primers for PCR and RT-PCR amplification of the different PERV subtypes, mtDNA , pocine β-actin and human GAPDH
| Gene | Primer sequence | Fragment size (bp) |
|---|---|---|
| PERV-A | F: 5′-TGGAAAGATTGGCAACAGCG-3′ | 360 |
| R: 5′-AGTGATGTTAGGCTCAGTGG-3′ | ||
| PERV-B | F: 5′-TTCTCCTTTGTCAATTCCGG-3′ | 264 |
| R: 5′-TACTTTATCGGGTCCCACTG-3′ | ||
| PERV-C | F: 5′-CTGACCTGGATTAGAACTGG-3′ | 281 |
| R: 5′-ATGTTAGAGGATGGTCCTGG-3′ | ||
| pol | F: 5′-CCACAGGGCAACGGCAGTATCC-3′ | 212 |
| R: 5′-TTGGAGGGTCAACACAGTGATGG-3′ | ||
| gag | F: 5′-CGGCAAGAGAAGAATTTGACT-3′ | 188 |
| R: 5′-CAGTTCCTTGCCCAGTGTCC-3′ | ||
| mtDNA | F: 5′-TCACCCATCATAGAAGAACTCCTACA-3′ | 281 |
| R: 5′-TTTTACGGTTAAGGCTGGGTTATTAAT-3′ | ||
| β-actin | F: 5′-CACGCCATCCTGCGTCTGGA-3′ | 100 |
| R: 5′-AGCACCGTGTTGGCGTAGAG-3′ | ||
| GAPDH | F: 5′-CAAGGTCATCCATGACAACTTTG-3′ | 496 |
| R: 5′-GTCCACCACCCTGTTGCTGTAG-3′ |
