Figure 6

MiR-320a and miR-140 play roles on TfR and MEV in a synergistic manner. (A) qPCR was used to assess effects of the miRNAs on the relative expression of TfR after 36 h transfection with the mimics, with β-actin as an internal control. (B) Western blot assay was used to assess the TfR protein levels of lysates of F81 cells after 48 h transfection with the miRNAs mimics, with β-actin as an internal control. (C) qPCR was used to assess the effects of the miRNAs on MEV genomic DNA at 12 h post-MEV infection, after 48 h transfection with the mimics. Data are from 3 independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test; *P <0.05; **P <0.01.